I need to do an equilibrium calc. of a bacteria growth medium to determine if precipitation between species occurs. I have started the input file using Solutions for each chemical (1M (NH4)2SO4, .1 M MgSO4, .2 M CaCl2, .1 M KH2PO4, .1 M Na2HPO4, .01 M FeSO4, 1 M HEPES (pH 7.2), .1 M Glucose), and then using MIX to combine all the solutions together in the appropriate amounts (ex: 2 mL (NH4)2SO4). The database already contains many of the compounds, although slightly different than I need, but I also need to define HEPES, glucose. CaCl2, etc. I have added Solution_Master_Species, but am confused with what I am to do there. I do not have much experience with these programs, so any input you could give me would be greatly appreciated. I have attached what I have so far. Thank you very much, Hanna Gilbert Hydrology and Water Resources University of Arizona
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